ABI5-mediated transactivation was inhibited by overexpression ofabi1-1, the dominant-negative allele of the protein phosphatase ABI1, and by 1-butanol, a competitive inhibitor of …

2019-06-01 · However, the overexpression of ABI5 in the bes1-D background could not rescue the early-flowering phenotype . Because plants overexpressing ABI5 decreased the ABA insensitivity of bes1-D , it is indicated that ABI3-mediated negative regulation of the floral transition would be independent of ABI5 activity, at least under higher-BR-response conditions.

7d), supporting the idea that NF-YC9 both facilitates ABI5 activity and interacts with other components such as RGL2 (Liu et al. 2016) to directly target to and stimulate ABI5 gene expression in response to ABA. 23 Oct 2015 To assess a physiological role for S-nitrosylation of the ABI5 protein during seed germination, ABI5- and ABI5Cys153Ser-overexpressing lines 9 Aug 2019 Overexpression of the soybean (Glycine max) GmbZIP1 gene improved high salt stress tolerance in transgenic plants (Gao et al. 2011), and a ABI5-mediated transactivation was inhibited by overexpression ofabi1-1, the dominant-negative allele of the protein phosphatase ABI1, and by 1-butanol, Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of 27 Feb 2014 In addition, HY5 binding to the ABI5 promoter is enhanced by ABA treatment, and overexpression of ABI5 restores ABA sensitivity in hy5 22 Apr 2017 (ABI5) in Arabidopsis. The AGL21-overexpressing plants were hypersensitive to ABA, salt, and osmotic stresses during seed germination and ABI5 phosphorylation by MAP kinases positively regulates ABI5 nuclear localization Overexpression of phosphor-null ABI5 in abi5-8 mutant restored the ABA Overexpression of ABI5/ABF binding proteins (AFPs) results in extreme ABA resistance of seeds via multiple mechanisms repressing ABA response, including 2020年9月10日 ABI5 Binding Proteins (AFPs) are a family of negative regulators of bZIP Induced by Transient Overexpression of Arabidopsis thaliana ABI5 ABI5 overexpression has been shown to resemble ABI3 overexpression in that it confers hypersensitivity to ABA inhibition of root growth and promotes slightly 3 Apr 2021 with ABI5 and appear to be involved in mediating stress responses. Induced by Transient Overexpression of Arabidopsis thaliana ABI5 6 Oct 2020 Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA- insensitive phe- notypes of coi1 mutants and JAZ-DJas (JAZ1, JAZ5, and 26 Sep 2000 Transgenic plants overexpressing ABI5 are hypersensitive to ABA, with respect to both germination and vegetative growth. We show that ABA ZFP3 overexpression in the Col-0, abi2-1, and abi4-. 101 backgrounds reduced hypocotyl lengths by.

Abi5 overexpression

Together, our results reveal a previously uncharacterized signaling module in which JAZ repressors of the JA pathway regulate the ABA-responsive ABI3 and ABI5 transcription factors to integrate JA and ABA signals during seed germination and post-germinative growth. ABI5 overexpression also results in high sensitivity to glucose and anthocyanin accumulation in response to sugar stress (Finkelstein et al., 2002). Analyses of transcript accumulation in abi5 mutants suggest that, similar to ABI3, ABI5 has both activator and repressor functions that may have either synergistic or antagonistic effects on gene expression, depending on the target gene. Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions. Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas exchange when plants experience periodic drought.

Conversely, overexpression of ABI5 enhanced light‐mediated hypocotyl inhibition in seedlings (Chen et al., 2008). Intriguingly, the regulatory interactions among light signalling factors while they modulate ABA signalling can sometimes be different from their primary interactions during light‐related developmental processes.

The overexpression of MdKNOX19 increased the ABA sensitivity of apple calli, resulting in a dramatic up-regulation in the transcription of the Arabidopsis ABI5 -like MdABI5 gene. However, overexpression of ABI5 was not sufficient to repress germination, as ABI5 activity requires phosphorylation.

Additionally, NF-YC9 up-regulates expression of the ABI5 gene in response to ABA. These findings show that NF-YC9 may be involved in ABA signaling as a positive regulator and likely functions redundantly together with other NF-YC members, and support the model that the NF-YC9 mediates ABA signaling via targeting to and aiding the ABA-responsive transcription factors such as ABI5.

Recent studies have shown that ABI5 is involved in photosynthesis by regulating chlorophyll metabolism [15,34,35]. To further analyze the function of ABI5 in chlorophyll metabolism, we treated the abi5-1 mutant and Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination Author: Bi, Chao, Ma, Yu, Wu, Zhen, Yu, Yong-Tao, Liang, Shan, Lu, Kai, Wang, Xiao-Fang Source: Plant molecular biology 2017 v.94 no.1-2 pp. 197-213 ISSN: 0167-4412 Subject: Overexpression of FOF2 results in delayed transitions to flowering under both LD and SD conditions.FOF2 expression is induced by ABA during seed germination where it acts through ABI3 and ABI5 … The bZIP transcription factor ABI5 is shown to confer an enhanced response to exogenous abscisic acid during germination, seedling establishment and subsequent vegetative growth of plants. ABI5 is necessary, but not sufficient, to maintain germinated embryos in a quiescent state, abscisic acid also being required for maintaining the quiescent state. ABI5 activity requires phosphorylation. The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line.

Overexpression of ABI5 reduces, whereas loss of function in the abi5 mutant increases, Cd accumulation in Arabidopsis, indicating the involvement of ABI5 in modulating Cd uptake.
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This protects plants from drought.

In the present study, we showed that inhibition of the catalase activity with 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of Col-0, abi5 mutants and ABI5-overexpression transgenic lines. ABI5 is necessary, but not sufficient, to maintain germinated embryos in a quiescent state, abscisic acid also being required for maintaining the quiescent state. ABI5 production is enhanced by stress including high salt and drought. This protects plants from drought.
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ABI5 is mainly expressed in dry seeds, and its expression markedly decreases after germination (Finkelstein and Lynch, 2000b; Lopez‐Molina et al., 2001). Expression of ABI5 is strongly induced by exogenous ABA (Lopez‐Molina et al., 2001).

Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas exchange when plants experience periodic drought. Noteworthily, we showed that down-regulation of NF-YC9 represses, but overexpression of NF-YC9 enhances, ABA-induced ABI5 expression (Fig. 7d), supporting the idea that NF-YC9 both facilitates ABI5 activity and interacts with other components such as RGL2 (Liu et al.

2019-08-09 · Background Basic region/leucine zippers (bZIPs) are transcription factors (TFs) encoded by a large gene family in plants. ABF3 and ABI5 are Group A bZIP TFs that are known to be important in abscisic acid (ABA) signaling. However, questions of whether ABF3 regulates ABI5 are still present. Results In vitro kinase assay results showed that Thr-128, Ser-134, and Thr-451 of ABF3 are calcium

2014-02-27 In addition, ABI5 also acts as an integrator for ABA and other plant hormone signals. Recent studies have shown that ABI5 is involved in photosynthesis by regulating chlorophyll metabolism [15,34,35]. To further analyze the function of ABI5 in chlorophyll metabolism, we treated the abi5-1 mutant and Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination Author: Bi, Chao, Ma, Yu, Wu, Zhen, Yu, Yong-Tao, Liang, Shan, Lu, Kai, Wang, Xiao-Fang Source: Plant molecular biology 2017 v.94 no.1-2 pp. 197-213 ISSN: 0167-4412 Subject: Overexpression of FOF2 results in delayed transitions to flowering under both LD and SD conditions.FOF2 expression is induced by ABA during seed germination where it acts through ABI3 and ABI5 … The bZIP transcription factor ABI5 is shown to confer an enhanced response to exogenous abscisic acid during germination, seedling establishment and subsequent vegetative growth of plants.

ABI5 production is enhanced by stress including high salt and drought. This protects plants from drought. However, overexpression of ABI5 was not sufficient to repress germination, as ABI5 activity requires phosphorylation. The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line. 2019-08-09 · Background Basic region/leucine zippers (bZIPs) are transcription factors (TFs) encoded by a large gene family in plants. ABF3 and ABI5 are Group A bZIP TFs that are known to be important in abscisic acid (ABA) signaling.