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Contents ©New England Biolabs Ltd. New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc. New England Biolabs, Inc. is an ISO 9001 certified company.

HDA reaction was carried out using an UvrD helicase   25 May 2020 The superfamily I helicases, UvrD and Rep are similar in structure and RecA (1 μM; from NEB) and 2 mM ATP were used for RecA assembly  5 Mar 2021 LAMP reaction, namely Tte UvrD helicase, which prevents unspecific 0.1 ng/ µl Tte UvrD Helicase (NEB, #M1202S), 0.05 U/µl thermostable  NEB® Golden Gate Assembly Kit (BsmBI-v2) · StickTogether™ DNA Ligase Buffer · NEBExpress MBP Tte-UvrD Helicase · Exo-CIP Rapid PCR Cleanup Kit   LAMP primers? Use the NEB LAMP Primer Design Tool. NEB's WarmStart LAMP Kit (DNA & RNA) offers speed and robust sensitivity Tte UvrD Helicase. What are Enzymes for Innovation? Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or  Tte-UvrD Helicase - 50 rxns NEB 5-alpha Competent E.coli (Subcloning Efficiency). 2.4 ml NEB Express Iq Competent E.coli (High Efficiency).

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Helicase-dependent Amplification Tte UvrD Helicase (NEB #M1202) • Thermostable • Improves specifically of problematic fluorescent LAMP reactions • High conc. Kontakt. New England Biolabs GmbH Brüningstr. 50; Geb. B852 D-65926 Frankfurt am Main Tel: +49-69/305-23140 E-Mail: info.de@neb.com 5'TCGA3'3'AGCT5'Thermo Scientific TaqI restriction enzyme recognizes T^CGA sites and cuts best at 65C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzyme Loop-mediated isothermal amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA. A strand-displacing DNA polymerase initiates synthesis and 2 of the primers form loop structures to facilitate subsequent rounds of amplification. Tte-UvrD Helicase - 50 rxns: Amazon.com: Industrial & Scientific.

Contents ©New England Biolabs Ltd. New England Biolabs Ltd. is the exclusive Canadian distributor for Cell Signaling Technology, Inc. New England Biolabs, Inc. is an ISO 9001 certified company.

FAQ: Does Tte UvrD Helicase (NEB #M1202) require any specific DNA structure for unwinding? No, Tte UvrD Helicase can utilize any dsDNA structure as a substrate for unwinding. This includes duplex DNA and duplexes with 3′and 5′ ssDNA overhangs (1-3).

11 Dec 2020 UvrD. Mfd. Mycobacterium tuberculosis. Mycobacterium smegmatis μl of proteinase K (P8107S; NEB) for 1.5 h at 60 °C. Samples were then 

Product Class: Other Tte UvrD Helicase Need assistance designing LAMP primers?

ZMO1484. UvrD/REP helicase T5 exonuclease ( NEB, USA), 0.5 μL buffer 4 (NEB, USA), and the final volume was set to 5 μL  Furthermore, Tte UvrD helicase (NEB) was predicted to improve the specificity of isothermal amplifications, particularly when using WarmStart LAMP kits [29, 30]. enzymes were purchased from NEB (Ipswich, USA). polymerase (NEB), and 1 × Phusion HF Buffer).
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2019-01-16 · WarmStart LAMP Master Mix (E1700, 2X) 12.5 μL LAMP Fluorescent Dye (50X) 0.5 μL Primers (10X) 2.5 μL* Tte UvrD Helicase : 0.5 – 1 μL (10 – 20 ng) DNA or RNA sample 2019-01-14 · FAQ: Does Tte UvrD Helicase (NEB #M1202) require any specific DNA structure for unwinding? No, Tte UvrD Helicase can utilize any dsDNA structure as a substrate for unwinding.

Product Introduction Unwinds double-stranded DNA Thermostable to 65°C Reduces non-specific product A: Enzymes for Innovation (EFI) is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications.
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240 County Road Ipswich, MA 01938-2723 978-927-5054 (Toll Free) 1-800-632-5227 Fax: 978-921-1350 Info@neb.com

www.neb.com info@neb.com M1202S / Lot: 10035793 Page 2 of 2 Assay Name/Specification Lot # 10035793 and a minimum of 1 µl of Tte-UvrD Helicase is incubated at 37°C. After incubation for 16 hours, >90% of the substrate RNA remains intact as determined by gel electrophoresis using fluorescent detection. Non-Specific DNase Activity (16 Hour) Protein Purity Assay (SDS-PAGE) - Tte-UvrD Helicase is · 95% pure as determined by SDS-PAGE analysis using Coomassie Blue detection. RNase Activity (Extended Digestion) - A 10 µl reaction in NEBuffer 4 containing 40 ng of a 300 base single-stranded RNA and a minimum of 1 µl of Tte-UvrD Helicase is incubated at 37°C. NEB ® TV Ep. 23 – Colorimetric LAMP in point-of-care diagnostics Learn the basics of Colorimetric LAMP, and how groups like NEB and the World Mosquito Program are using this isothermal amplification method in field tests and point-of-care diagnostics.

FAQ: Is Tte UvrD Helicase compatible with the WarmStart Colorimetric LAMP 2X Master Mix (NEB #M1800)? No, Tte UvrD Helicase should not be used with Colorimetric LAMP. As the UvrD acts on DNA substrates it hydrolyzes dATP, releasing an H+ with each hydrolysis much like a DNA polymerase incorporating dNTP.

Object Moved This document may be found here UvrD and UvrD K708A were purified from BL21(DE3) cells transformed with pETDUET-UvrD or pETDUET-UvrD K708A, which was made from pETDUET-UvrD by site directed mutagenesis.

Are you doing COVID-19 related research? Our new RUO kit, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit, enables simple, visual detection of isothermal amplification of SARS-CoV-2 nucleic acid. The LAMP Fluorescent Dye is an intercalating dye that can be used to track a LAMP reaction in real-time by fluorescence measurement. It is provided at 50X concentration and includes an amount sufficient for at least 500 LAMP reactions (0.5 µl per 25 µl LAMP reaction is recommended when monitoring reactions on most real time qPCR instruments). Object Moved This document may be found here UvrD and UvrD K708A were purified from BL21(DE3) cells transformed with pETDUET-UvrD or pETDUET-UvrD K708A, which was made from pETDUET-UvrD by site directed mutagenesis. Cells were grown at 37°C to an A 600 of ∼0.5 before being used to inoculate 1 l of LB + 100 μg/ml ampicillin to an A 600 of ∼0.025. ニュー・イングランド・バイオラボ －nebは制限酵素、エンドヌクレアーゼ、リコンビナント酵素、pcr用試薬、発現システム、マーカー、コンピテントセル、rna研究用試薬、ポリメラーゼ、修飾酵素、核酸、細胞解析などの試薬を提供しています。 www.neb.com info@neb.com M1202S / Lot: 10062300 Page 2 of 2 Assay Name/Specification Lot # 10062300 Exonuclease Activity (Radioactivity Release) A 50 µl reaction in CutSmart® Buffer containing 1 µg of a mixture of single and double-stranded [ ³H] E. coli DNA and a minimum of 2 µg of Tte-UvrD Helicase Tte UvrD Helicase WarmStart ® Colorimetric LAMP 2X Master Mix (DNA & RNA) WarmStart ® Colorimetric LAMP 2X Master Mix with UDG WarmStart ® LAMP Kit (DNA & RNA) WarmStart® RTx Reverse Transcriptase Whole Genome Amplification & Multiple Displacement Amplification Deoxynucleotide (dNTP) Solution Mix phi29 DNA Polymerase Pyrophosphatase The NEB catalog highlights a wide variety of enzyme functionalities found in nature or engineered for specific purposes Are you doing COVID-19 related research?